Tissue Science & Regenerative Medicine

Biography

Biography: Joel I. Osorio

Abstract

As it has been previously demonstrated that co-electroporation of Xenopus laevis frog oocytes with normal cells and cancerous cell lines in-duces the expression of pluripotency markers, and in experimental murine model studies that mRNA extract (Bioquantine® purified from in-tra- and extra-oocyte liquid phases of electropo-rated oocytes) showed potential as a treatment for a wide range of conditions as Squint, Spinal Cord Injury (SCI) and Cerebral Palsy among others. The current study observed beneficial changes with Bioquantine administration in a patient with a severe SCI. Pluripotent stem cells have therapeutic and regenerative potential in clinical situations CNS disorders even cancer. One method of reprogramming somatic cells into pluripotent stem cells is to expose them to extracts prepared from Xenopus laevis oocytes We showed previously that coelectroporation of Xenopus laevis frog oocytes; with normal cells and cancerous cells lines, induces expression of markers of  pluripotency.We also observed ther-apeutic effects of treatment with a purified ex-tract (Bioquantine) of intra- and extra-oocyte liquid phases derived from electroporated X. laevis oocytes, on experimentally induced pathologies including murine models of melanoma, traumatic brain injury, and experi-mental skin wrinkling induced by squalene-monohydroperoxide (Paylian et al, 2016). The positive human findings for Spinal Cord Injury, and Cerebral Palsy with the results from previ-ous animal studies with experimental models of traumatic brain injury, respectively (Paylian et al, 2016). Because of ethical reasons, legal re-strictions, and a limited numbers of patients, we were able to treat only a very small number of patients. These results indicate that Bioquan-tine® may be safe and well tolerated for use in humans, and deserves further study in a range of degenerative disorders. We propose that the mechanism of action of Bioquantine® in these various diseases derives from its unique phar-macology and combinatorial reprogramming properties. In conclusion, these preliminary find-ings suggest that Bioquantine is safe and well tolerated on patients with Cerebral Palsy and-Spinal Cord Injury, among others. In addition to the regenerative therapy and due to the patient condition, we decided to include the Restore-Sensor SureScan^5-6 . Based on the of electrical stimulation for rehabilitation and regeneration after spinal cord injury published by Hamid and MacEwan , we designed an improved deliv-ery method for the in situ application of MSCs and Bioquantine in combination with the RestoreSensor SureScan Conclusions: To the present day the patient who suffered a total sec-tion of spinal cord at T12-L1 shows an im-provement in sensitivity, strength in striated muscle and smooth muscle connection, 11 months after the first therapy of cell regeneration and 3 month after the placement of RestoreSen-sor at the level of the lesion, the patient with a complete medullary section shows an evident improvement on his therapy of physical rehabili-tation on crawling from front to back by himself and standing on his feet for the first time and showing a progressively important functionality on the gluteal and legs sensitivity.