9th International Conference & Exhibition on Tissue Science & Regenerative Medicine April 23-24, 2018 Las Vegas, Nevada, USA

Biography:

Dr. Osorio is an innovative businessman with a distinct entrepreneurial mindset concentrated adding value on areas of Biotechnology (mRNA), Reprogramming & Regenerative Medicine for translational use in humans and a variety of clinical applications aimed for both the private and the public health sectors.

Abstract:

As it has been previously demonstrated that co-electroporation of Xenopus laevis frog oocytes with normal cells and cancerous cell lines in-duces the expression of pluripotency markers, and in experimental murine model studies that mRNA extract (Bioquantine® purified from in-tra- and extra-oocyte liquid phases of electropo-rated oocytes) showed potential as a treatment for a wide range of conditions as Squint, Spinal Cord Injury (SCI) and Cerebral Palsy among others. The current study observed beneficial changes with Bioquantine administration in a patient with a severe SCI. Pluripotent stem cells have therapeutic and regenerative potential in clinical situations CNS disorders even cancer. One method of reprogramming somatic cells into pluripotent stem cells is to expose them to extracts prepared from Xenopus laevis oocytes We showed previously that coelectroporation of Xenopus laevis frog oocytes; with normal cells and cancerous cells lines, induces expression of markers of  pluripotency.We also observed ther-apeutic effects of treatment with a purified ex-tract (Bioquantine) of intra- and extra-oocyte liquid phases derived from electroporated X. laevis oocytes, on experimentally induced pathologies including murine models of melanoma, traumatic brain injury, and experi-mental skin wrinkling induced by squalene-monohydroperoxide (Paylian et al, 2016). The positive human findings for Spinal Cord Injury, and Cerebral Palsy with the results from previ-ous animal studies with experimental models of traumatic brain injury, respectively (Paylian et al, 2016). Because of ethical reasons, legal re-strictions, and a limited numbers of patients, we were able to treat only a very small number of patients. These results indicate that Bioquan-tine® may be safe and well tolerated for use in humans, and deserves further study in a range of degenerative disorders. We propose that the mechanism of action of Bioquantine® in these various diseases derives from its unique phar-macology and combinatorial reprogramming properties. In conclusion, these preliminary find-ings suggest that Bioquantine is safe and well tolerated on patients with Cerebral Palsy and-Spinal Cord Injury, among others. In addition to the regenerative therapy and due to the patient condition, we decided to include the Restore-Sensor SureScan^5-6 . Based on the of electrical stimulation for rehabilitation and regeneration after spinal cord injury published by Hamid and MacEwan , we designed an improved deliv-ery method for the in situ application of MSCs and Bioquantine in combination with the RestoreSensor SureScan Conclusions: To the present day the patient who suffered a total sec-tion of spinal cord at T12-L1 shows an im-provement in sensitivity, strength in striated muscle and smooth muscle connection, 11 months after the first therapy of cell regeneration and 3 month after the placement of RestoreSen-sor at the level of the lesion, the patient with a complete medullary section shows an evident improvement on his therapy of physical rehabili-tation on crawling from front to back by himself and standing on his feet for the first time and showing a progressively important functionality on the gluteal and legs sensitivity.

Biography:

Abstract:

Bone microenvironment is a complex milieu composed of inorganic and organic components.  In addition to its mechanical and chemical role this microenvironment  gives rise to heterogonous molecules and cells that in many cases interacting in an orchestrated manner and control signaling pathways that enable bone development and maintenance. Solid cancers originating in the breast, prostate, and lung tend to metastasize to bone. Once deployed in bone these tumor cells harness this microenvironment, shift to a quiescent mode or initiate a vicious cycle that often leads bone destruction and gain an increased tumorigenicity by mechanisms which are not yet fully understood. Here we introduce a new three-dimensional model which closely resembles a living natural bone that can be used to study cellular and molecular cues in bone tumors and metastasis. Using this model we showed that the mineral phase may have an important role on cellular characteristics such as, proliferation rates and tumorigenicity. We also revealed that interactions with mesenchymal stem cells (MSC's) increased migration and invasion capacities along with osteosarcomas (OS) proliferation, moreover we showed that via regulation of pathways such Wnt, cadherins, Notch and their downstream target genes such as c-Myc, these capacities were further enhanced when accommodated with the bone like biolattice and directly interacted with the MSCs. We also suggest that progression in OS aggressiveness can also can be attributed to a transition in Wnt signaling from canonical to noncanonical pathways, which is intensified in presence of MSCs. We suggest these kind of tumor promoting interactions may be found in the natural and tumorigenic bone microenvironment. New insights on the interplay between these signaling cues and their effects tumor progression will be discussed. A better understanding of the molecular signaling mechanisms involved in the tumor development and bone metastasis may contribute to development of new cancer therapies. 

Biography:

Ye-Eun Yoon received her MS degree in the Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul, Korea. Her Master’s thesis is entitled “Development of adhesive/multi-layered scaffolds for tissue adhesives and gene delivery vehicles”. She has already published several peer-reviewed research papers in reputed journals and won an American Chemical Society (ACS) Editors’ Choice Award in 2016. Currently, she works as a research scientist at Research & Development Center, Cosmocos Corporation, a branch of Korea Tomorrow and Global (KT&G), in Korea

Abstract:

Marine mussels produce and secrete adhesive proteins that allow themselves to attach in rough marine environments. Currently, six mussel adhesive proteins (fp-1 through fp-6) have been identified from the adhesive plaques of mussels. Mussel adhesive proteins have been considered as a desirable source of water-resistant bioadhesives which can also be used as a useful ingredient in cosmetics. This study aimed to construct a recombinant hybrid mussel adhesive protein, fp-13151, in order to get convenient and economical production and enhance the adhesive capability of the mussel proteins, and evaluate its potential as a functional cosmetic ingredient. The hybrid gene was constructed to contain the parts of fp-1/fp-3/fp-1/fp-5/fp-1in a sequential order using recombinant DNA techniques. fp-13151 was purified from the corresponding overexpressed E. coli cells using affinity chromatography. fp-13151 did not exhibit cytotoxicity and irritability to the skin. fp-13151 at 50 μg/ml augmented the synthesis of collagen 1.63-fold over that of the non-treated control in HaCaT cells, implying its anti-wrinkle activity. On the contrary, fp-13151 diminished the levels of matrix metalloproteinase (MMP-1), also known as interstitial collagenase, in a concentration-dependent manner. It could marginally inhibit elastase activity in an in vitro experiment. fp-13151 was able to inhibit both monooxygenase and oxidase activities of mushroom tyrosinase in a concentration-dependent manner, suggesting its skin whitening activity. It was also found to contain an antioxidant activity, when an ABTS radical scavenging capacity assay was used. In a pilot-scale clinical trial, the essence containing fp-13151 significantly reduced the wrinkle parameters tested in the participants after both 4- and 8-week treatment, compared to the control group. Taken together, fp-13151 possesses skin beneficial properties, such as antioxidant, whitening and anti-wrinkle activities, in addition to its peculiar adhesive character. These findings suggest that fp-13151 has a potential as an effective ingredient in the manufacture of functional cosmetics.

Biography:

Andisheh Ghashghaie is expert in bioprocessing and cryopreservation of cord blood units. She is also skilled in expansion and culture of mesenchymal stromal cells which are then used for GVHD treatment and regenerative medicine. She has worked in Pasteur institute of Iran as a master student and has more than 10 years experience in HLA typing especially by PCR-SSP method. She has become self-made by years of experience in research, training and supervising in hospital, BMT laboratory and Stem Cell Research Center. Hematology_Oncology & Stem Cell Transplantation institute is been founded 25 years ago and been performing/running SC transplants for both malignant and non-malignant patients from around the country. GVHD as the major life-threatening result of engraftment is the most studied risk factor under several research projects by academic members, master and Ph.D. students and fellowships in this institute. This study has been focusing on quality of Cord blood units and their utilization in SC transplant aftermaths.

Abstract:

Collection and banking of umbilical cord blood (UCBs) can provide unlimited source of ethnically diverse donors. The main limitation factor for use of (UCBs) as a source of hematopoietic progenitors for transplantation is cell dose. The engraftment outcome of UCB transplantation is highly dependent on nucleated cell number of unites. It would be useful to predict CB cell content using information of donor-related variables before cell processing.

Banked unrelated donor UCBs has improved access to hematopoietic stem cell transplantation for patients without a suitably matched donor. In a resource-limited environment, ensuring that the public inventory is enriched with high-quality (CBUs) addressing the needs of a diverse group of patients is a priority. Identification of donor characteristics correlating with higher CBU quality could guide operational strategies to increase the yield of banked high-quality CBUs.

In contrast family-directed CB collection and storage which requires different procedures in order to obtain high-quality products. This approach is clinically indicated and validated in families where the mother is pregnant and has an existing child or has a known risk of having a child affected by a disease which can be cured by allogeneic HSCT. It would be useful to predict CB cell content using information of donor-related variables before processing.

In this study, CBs were obtained from 3297 single-birth term deliveries in 3 hospitals affiliated to Tehran University of medical sciences from January 1998 to June 2016. Up to August 2016, 67 units have been used in transplantation for patients with malignant and non-malignant disorders. The attempt has been made to find factors which have significant effects on quality of CB units, including CB volume, TNCs, and CD34+ cell counts.